{"id":754,"date":"2024-04-25T22:00:46","date_gmt":"2024-04-25T22:00:46","guid":{"rendered":"https:\/\/winterdev.cc\/nwgc-dev\/?page_id=754"},"modified":"2026-03-04T17:41:39","modified_gmt":"2026-03-04T17:41:39","slug":"sequencing","status":"publish","type":"page","link":"https:\/\/nwgc.gs.washington.edu\/wordpress\/services\/functional-genomics\/fiberseq\/sequencing\/","title":{"rendered":"Sequencing – Available Platforms"},"content":{"rendered":"

Typically, 350 million paired-end reads are needed for each scATAC-seq library and 250 million paired-end reads for each scRNA-seq library. Depending on how many libraries are pooled together, the kit size will vary.<\/p>\n

\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Sequencing Platform<\/strong><\/td>\nCycles (bp)<\/strong><\/td>\nNumber of Reads
*paired-end<\/strong><\/td>\n<\/tr>\n
IIllumina\u00ae i100 MiSeq 5M<\/td>\n300, 500<\/td>\n~10 million<\/td>\n<\/tr>\n
Illumina\u00ae i100 MiSeq 25M<\/td>\n100, 300, 600, 1000<\/td>\n~50 million<\/td>\n<\/tr>\n
Illumina\u00ae i100 MiSeq 50M<\/td>\n100, 300, 600<\/td>\n~100 million<\/td>\n<\/tr>\n
IIllumina\u00ae i100 MiSeq 100M<\/td>\n100, 300<\/td>\n~200 million<\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq 6000 SP<\/td>\n100, 200, 300<\/td>\n~1.6 billion  <\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq 6000 S1<\/td>\n100, 200, 300<\/td>\n~3.2 billion<\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq 6000 S2<\/td>\n100, 200, 300<\/td>\n~8.2 billion<\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq 6000 S4<\/td>\n35, 200, 300<\/td>\n~20 billion<\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq X Plus 1.5B<\/td>\n100, 200, 300<\/td>\n~3 billion<\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq X Plus 10B<\/td>\n100, 200, 300<\/td>\n~20 billion<\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq X Plus 25B<\/td>\n300<\/td>\n~50 billion<\/td>\n<\/tr>\n
Element AVITI24\u2122 Cloudbreak Freestyle Low Output<\/td>\n150, 300<\/td>\n~150 million for 150 cycle
~375 million for 300 cycle<\/td>\n<\/tr>\n
Element AVITI24\u2122 Cloudbreak Freestyle Mid Output<\/td>\n150, 300, 600<\/td>\n~750 million
*600 cycle kit ~100 million<\/td>\n<\/tr>\n
Element AVITI24\u2122 Cloudbreak Freestyle High Output<\/td>\n150, 300, 600<\/td>\n~1.5 billion
*600 cycle kit ~300 million<\/td>\n<\/tr>\n
Element AVITI24\u2122 Cloudbreak Freestyle UltraQ<\/td>\n300<\/td>\n~800 million<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n","protected":false},"excerpt":{"rendered":"

Typically, 350 million paired-end reads are needed for each scATAC-seq library and 250 million paired-end reads for each scRNA-seq library. Depending on how many libraries are pooled together, the kit size will vary. Sequencing Platform Cycles (bp) Number of Reads*paired-end IIllumina\u00ae i100 MiSeq 5M 300, 500 ~10 million Illumina\u00ae i100 MiSeq 25M 100, 300, 600, 1000 ~50 million Illumina\u00ae i100 MiSeq 50M 100, 300, 600 ~100 million IIllumina\u00ae i100 MiSeq 100M 100, 300 ~200 million Illumina\u00ae NovaSeq 6000 SP 100,…<\/p>\n

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