{"id":477,"date":"2024-04-25T20:23:17","date_gmt":"2024-04-25T20:23:17","guid":{"rendered":"https:\/\/winterdev.cc\/nwgc-dev\/?page_id=477"},"modified":"2026-03-04T17:32:43","modified_gmt":"2026-03-04T17:32:43","slug":"sequencing","status":"publish","type":"page","link":"https:\/\/nwgc.gs.washington.edu\/wordpress\/services\/already-prepped-libraries\/sequencing\/","title":{"rendered":"Available Platforms"},"content":{"rendered":"
\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Sequencing Platform<\/strong><\/td>\nCycles (bp)<\/strong><\/td>\nNumber of Reads
*paired-end<\/strong><\/td>\n<\/tr>\n
Illumina\u00ae i100 MiSeq 5M<\/td>\n300, 500<\/td>\n~10 million<\/td>\n<\/tr>\n
Illumina\u00ae i100 MiSeq 25M<\/td>\n100, 300, 600, 1000<\/td>\n~50 million<\/td>\n<\/tr>\n
Illumina\u00ae i100 MiSeq 50M<\/td>\n100, 300, 600<\/td>\n~100 million<\/td>\n<\/tr>\n
Illumina\u00ae i100 MiSeq 100M<\/td>\n100, 300<\/td>\n~200 million<\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq 6000 SP<\/td>\n100, 200, 300<\/td>\n~1.6 billion  <\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq 6000 S1<\/td>\n100, 200, 300<\/td>\n~3.2 billion<\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq 6000 S2<\/td>\n100, 200, 300<\/td>\n~8.2 billion<\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq 6000 S4<\/td>\n35, 200, 300<\/td>\n~20 billion<\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq X Plus 1.5B<\/td>\n100, 200, 300, (600 soon!)<\/td>\n~3 billion<\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq X Plus 5B (coming soon!)<\/td>\n100, 200, 300, 600<\/td>\n~10 billion<\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq X Plus 10B<\/td>\n100, 200, 300<\/td>\n~20 billion<\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq X Plus 25B<\/td>\n300<\/td>\n~50 billion<\/td>\n<\/tr>\n
Element AVITI24\u2122 Cloudbreak Freestyle Low Output<\/td>\n150, 300<\/td>\n~150 million for 150 cycle
~275 million for 300 cycle<\/td>\n<\/tr>\n
Element AVITI24\u2122 Cloudbreak Freestyle Mid Output<\/td>\n150, 300, 600<\/td>\n~750 million
*600 cycle kit ~100 million<\/td>\n<\/tr>\n
Element AVITI24\u2122 Cloudbreak Freestyle High Output<\/td>\n150, 300, 600<\/td>\n~1.5 billion
*600 cycle kit ~300 million<\/td>\n<\/tr>\n
Element AVITI24\u2122 Cloudbreak Freestyle UltraQ<\/td>\n300<\/td>\n~800 million<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n
\n\n\n\n\n\n
Long-Read Sequencing Platform<\/strong><\/td>\nFlowcell Minimum<\/strong><\/td>\nOutput (Gb)<\/strong><\/td>\n<\/tr>\n
PacBio Revio\u2122<\/td>\n1 SMRTcell
(Run Time: 12, 24, or 30hrs)<\/td>\n		~60-90<\/td>\n<\/tr>\n
Oxford Nanopore Technologies\u00ae<\/td>\n1 flowcell<\/td>\n~50-150<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n","protected":false},"excerpt":{"rendered":"

Sequencing Platform Cycles (bp) Number of Reads*paired-end Illumina\u00ae i100 MiSeq 5M 300, 500 ~10 million Illumina\u00ae i100 MiSeq 25M 100, 300, 600, 1000 ~50 million Illumina\u00ae i100 MiSeq 50M 100, 300, 600 ~100 million Illumina\u00ae i100 MiSeq 100M 100, 300 ~200 million Illumina\u00ae NovaSeq 6000 SP 100, 200, 300 ~1.6 billion   Illumina\u00ae NovaSeq 6000 S1 100, 200, 300 ~3.2 billion Illumina\u00ae NovaSeq 6000 S2 100, 200, 300 ~8.2 billion Illumina\u00ae NovaSeq 6000 S4 35, 200, 300 ~20 billion Illumina\u00ae…<\/p>\n

Continue reading Available Platforms<\/span><\/a><\/div>\n","protected":false},"author":2,"featured_media":0,"parent":306,"menu_order":5,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-477","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/477","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/comments?post=477"}],"version-history":[{"count":3,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/477\/revisions"}],"predecessor-version":[{"id":2472,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/477\/revisions\/2472"}],"up":[{"embeddable":true,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/306"}],"wp:attachment":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/media?parent=477"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}