Thank you for your interest in the NWGC!<\/p>\n
Below is the step-by-step process for getting your already constructed libraries sequenced. Please review these steps carefully and if you have any questions, please don’t hesitate to ask.<\/p>\n
To request a quote, please email nwgc@uw.edu<\/a> with the following information:<\/p>\n Investigator Name *Request Information should include as much detail as possible about your project requirements. For guidance, please see the Getting Started pages under your project type for more information.<\/p>\n The NWGC will send you a quote based on your project specifications. Do not send any samples before reviewing the quote and returning the signed quote or purchase order to the NWGC.<\/p>\n Once a quote is received and signed, please email the NWGC Sequencing Team at nwgcseq@uw.edu<\/a> to receive the Sample Submission Form. Please fully fill out the form and return it to nwgcseq@uw.edu<\/a> prior to sample drop-off or delivery. Please also send all the barcode information for your library(s).<\/p>\n **We use the information provided to load the sequencing instruments and generate FASTQs. Please make sure it is as accurate as possible.<\/p>\n Please follow the guidelines for submission below:<\/p>\n Short-Read Specific: <\/em><\/p>\n Long-Read Specific:<\/em><\/p>\n All short-read sequencing samples will be run on the Bioanalyzer and Qubit. All long-read sequencing samples will be run on the Femto and Qubit. QC results will be emailed to the investigator before sequencing.<\/p>\n Globus is a service provider that manages login credentials and coordinates transfers between organizations. Please visit the\u00a0Data Delivery<\/em>\u00a0section for more information.<\/p>\n Once your run is complete, we will send you an email with a link to your specific Globus folder where the run file is located.<\/p>\n Thank you for your interest in the NWGC! Below is the step-by-step process for getting your already constructed libraries sequenced. Please review these steps carefully and if you have any questions, please don’t hesitate to ask. 1.) Request a Quote To request a quote, please email nwgc@uw.edu with the following information: Investigator NamePhone NumberEmail AddressRequest Information *Request Information should include as much detail as possible about your project requirements. For guidance, please see the Getting Started pages under your project type for…<\/p>\n
Phone Number
Email Address
Request Information<\/p>\n2.) Sample Submission Information<\/h3>\n
Required Sample Information:<\/h3>\n
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4.) QC Results<\/h3>\n
5.) Data Delivery\/Globus Access<\/h3>\n
\nSupplemental Info:<\/h2>\n
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\n Sequencing Platform<\/strong><\/td>\n Minimum Molarity<\/strong><\/td>\n Minimum Volume<\/strong><\/td>\n<\/tr>\n \n Illumina\u00ae NovaSeq S4<\/td>\n 5nM<\/td>\n 130uL<\/td>\n<\/tr>\n \n Illumina\u00ae NovaSeq S2<\/td>\n 5nM<\/td>\n 55uL<\/td>\n<\/tr>\n \n Illumina\u00ae NovaSeq SP\/S1<\/td>\n 5nM<\/td>\n 35uL<\/td>\n<\/tr>\n \n Illumina\u00ae NovaSeq X Plus 1.5B<\/td>\n 5nM<\/td>\n 20uL<\/td>\n<\/tr>\n \n Illumina\u00ae NovaSeq X Plus 10B<\/td>\n 5nM<\/td>\n 60uL<\/td>\n<\/tr>\n \n Illumina\u00ae NovaSeq X Plus 25B<\/td>\n 5nM<\/td>\n 100uL<\/td>\n<\/tr>\n \n Illumina\u00ae NextSeq 500 MO or HO<\/td>\n 5nM<\/td>\n 10uL<\/td>\n<\/tr>\n \n Illumina\u00ae MiSeq<\/td>\n 4nM<\/td>\n 20uL<\/td>\n<\/tr>\n \n Element AVITI\u2122 Cloudbreak Freestyle<\/td>\n 5nM<\/td>\n 20uL<\/td>\n<\/tr>\n<\/tbody>\n<\/table> \n\n
\n Long-Read Sequencing Platform<\/strong><\/td>\n Minimum Concentration<\/strong><\/td>\n Minimum order<\/strong><\/td>\n<\/tr>\n \n PacBio Revio\u2122<\/td>\n 20-60ng\/ul in 28ul (concentration varies based on insert size)<\/td>\n SMRT\u2122 Cell<\/td>\n<\/tr>\n \n Oxford Nanopore Technologies\u00ae<\/td>\n 2-10ug (concentration varies based on prep type and coverage needed)<\/td>\n Flow cell<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n \n