{"id":459,"date":"2024-04-25T20:20:56","date_gmt":"2024-04-25T20:20:56","guid":{"rendered":"https:\/\/winterdev.cc\/nwgc-dev\/?page_id=459"},"modified":"2024-04-25T20:20:56","modified_gmt":"2024-04-25T20:20:56","slug":"library-prep","status":"publish","type":"page","link":"https:\/\/nwgc.gs.washington.edu\/wordpress\/services\/structural-genomics\/long-read\/ont\/library-prep\/","title":{"rendered":"Library Prep"},"content":{"rendered":"<p><a href=\"https:\/\/nanoporetech.com\/products\/prepare\/dna-library-preparation\">Ligation Sequencing Kit or Ultra-long DNA Sequencing Kit<\/a><\/p>\n<p>*open to testing and using other prep kits<\/p>\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"930\" height=\"500\" src=\"https:\/\/winterdev.cc\/nwgc-dev\/wp-content\/uploads\/2024\/07\/image-13.png\" alt=\"\" class=\"wp-image-999\" style=\"width:605px;height:auto\" srcset=\"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-content\/uploads\/2024\/07\/image-13.png 930w, https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-content\/uploads\/2024\/07\/image-13-300x161.png 300w, https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-content\/uploads\/2024\/07\/image-13-768x413.png 768w, https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-content\/uploads\/2024\/07\/image-13-375x202.png 375w\" sizes=\"auto, 100vw\" \/><\/figure>\n<\/p>\n<p>For high molecular weight (HMW) DNA (for ultralong), samples are extracted using the <a href=\"https:\/\/www.neb.com\/en-us\/products\/t3050-monarch-hmw-dna-extraction-kit-for-cells-and-blood\">NEB Monarch Kits<\/a> (cells\/blood or tissue.)<\/p>\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"800\" height=\"450\" src=\"https:\/\/winterdev.cc\/nwgc-dev\/wp-content\/uploads\/2024\/07\/image-14.png\" alt=\"\" class=\"wp-image-1005\" style=\"width:342px;height:auto\" srcset=\"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-content\/uploads\/2024\/07\/image-14.png 800w, https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-content\/uploads\/2024\/07\/image-14-300x169.png 300w, https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-content\/uploads\/2024\/07\/image-14-768x432.png 768w, https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-content\/uploads\/2024\/07\/image-14-375x211.png 375w\" sizes=\"auto, 100vw\" \/><\/figure><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Ligation Sequencing Kit or Ultra-long DNA Sequencing Kit *open to testing and using other prep kits For high molecular weight (HMW) DNA (for ultralong), samples are extracted using the NEB Monarch Kits (cells\/blood or tissue.)<\/p>\n<div><a class=\"more-link\" href=\"https:\/\/nwgc.gs.washington.edu\/wordpress\/services\/structural-genomics\/long-read\/ont\/library-prep\/\">Continue reading <span class=\"screen-reader-text\">Library Prep<\/span><\/a><\/div>\n","protected":false},"author":2,"featured_media":0,"parent":335,"menu_order":4,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-459","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/459","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/comments?post=459"}],"version-history":[{"count":0,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/459\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/335"}],"wp:attachment":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/media?parent=459"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}