{"id":457,"date":"2024-04-25T20:20:42","date_gmt":"2024-04-25T20:20:42","guid":{"rendered":"https:\/\/winterdev.cc\/nwgc-dev\/?page_id=457"},"modified":"2024-04-25T20:20:42","modified_gmt":"2024-04-25T20:20:42","slug":"quality-control","status":"publish","type":"page","link":"https:\/\/nwgc.gs.washington.edu\/wordpress\/services\/structural-genomics\/long-read\/ont\/quality-control\/","title":{"rendered":"Quality Control"},"content":{"rendered":"<p>The Northwest Genomics Center (NWGC) performs multiple QC steps on DNA and RNA samples prior to library prep and sequencing.<\/p>\n<h3 class=\"wp-block-heading\">Concentration Validation<\/h3>\n<p>DNA samples are tested using the Quant-iT High Sensitivity dsDNA kit, using the Qubit Flex, to measure concentration. RNA samples are tested using the Quant-iT RNA kit to measure concentration and we require RIN\/RQN\u2019s reported by users.<\/p>\n<h3 class=\"wp-block-heading\">Insert Size<\/h3>\n<p>All samples are run on the Femto for insert size analysis.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The Northwest Genomics Center (NWGC) performs multiple QC steps on DNA and RNA samples prior to library prep and sequencing. Concentration Validation DNA samples are tested using the Quant-iT High Sensitivity dsDNA kit, using the Qubit Flex, to measure concentration. RNA samples are tested using the Quant-iT RNA kit to measure concentration and we require RIN\/RQN\u2019s reported by users. Insert Size All samples are run on the Femto for insert size analysis.<\/p>\n<div><a class=\"more-link\" href=\"https:\/\/nwgc.gs.washington.edu\/wordpress\/services\/structural-genomics\/long-read\/ont\/quality-control\/\">Continue reading <span class=\"screen-reader-text\">Quality Control<\/span><\/a><\/div>\n","protected":false},"author":2,"featured_media":0,"parent":335,"menu_order":3,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-457","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/457","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/comments?post=457"}],"version-history":[{"count":0,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/457\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/335"}],"wp:attachment":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/media?parent=457"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}