{"id":427,"date":"2024-04-25T20:16:08","date_gmt":"2024-04-25T20:16:08","guid":{"rendered":"https:\/\/winterdev.cc\/nwgc-dev\/?page_id=427"},"modified":"2024-04-25T20:16:08","modified_gmt":"2024-04-25T20:16:08","slug":"library-prep","status":"publish","type":"page","link":"https:\/\/nwgc.gs.washington.edu\/wordpress\/services\/structural-genomics\/short-read\/wes\/library-prep\/","title":{"rendered":"Library Prep"},"content":{"rendered":"<p>Exome sequencing libraries are constructed using the KAPA HTP Library Preparation kit and captured using the Twist Biosciences Human Core Exome supplemented with additional probes selected by the NWGC to robustly cover all regions of the exome.  The NWGC also works with investigators to design and synthesize custom panels with Twist Biosciences.<\/p>\n<p><a href=\"https:\/\/www.twistbioscience.com\">Twist Human Core Exome Kit + RefSeq<\/a><\/p>\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"800\" height=\"450\" src=\"https:\/\/winterdev.cc\/nwgc-dev\/wp-content\/uploads\/2024\/06\/image-5.png\" alt=\"\" class=\"wp-image-858\" style=\"width:487px;height:auto\" srcset=\"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-content\/uploads\/2024\/06\/image-5.png 800w, https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-content\/uploads\/2024\/06\/image-5-300x169.png 300w, https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-content\/uploads\/2024\/06\/image-5-768x432.png 768w, https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-content\/uploads\/2024\/06\/image-5-375x211.png 375w\" sizes=\"auto, 100vw\" \/><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>Exome sequencing libraries are constructed using the KAPA HTP Library Preparation kit and captured using the Twist Biosciences Human Core Exome supplemented with additional probes selected by the NWGC to robustly cover all regions of the exome. The NWGC also works with investigators to design and synthesize custom panels with Twist Biosciences. Twist Human Core Exome Kit + RefSeq<\/p>\n<div><a class=\"more-link\" href=\"https:\/\/nwgc.gs.washington.edu\/wordpress\/services\/structural-genomics\/short-read\/wes\/library-prep\/\">Continue reading <span class=\"screen-reader-text\">Library Prep<\/span><\/a><\/div>\n","protected":false},"author":2,"featured_media":0,"parent":328,"menu_order":4,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-427","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/427","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/comments?post=427"}],"version-history":[{"count":0,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/427\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/328"}],"wp:attachment":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/media?parent=427"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}