{"id":413,"date":"2024-04-25T20:14:19","date_gmt":"2024-04-25T20:14:19","guid":{"rendered":"https:\/\/winterdev.cc\/nwgc-dev\/?page_id=413"},"modified":"2026-03-04T17:31:41","modified_gmt":"2026-03-04T17:31:41","slug":"sequencing","status":"publish","type":"page","link":"https:\/\/nwgc.gs.washington.edu\/wordpress\/services\/structural-genomics\/short-read\/wgs\/sequencing\/","title":{"rendered":"Sequencing – Available Platforms"},"content":{"rendered":"
\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Sequencing Platform<\/strong><\/td>\nCycles (bp)<\/strong><\/td>\nNumber of Reads
*paired-end<\/strong><\/td>\n<\/tr>\n
IIllumina\u00ae i100 MiSeq 5M<\/td>\n300, 500<\/td>\n~10 million<\/td>\n<\/tr>\n
Illumina\u00ae i100 MiSeq 25M<\/td>\n100, 300, 600, 1000<\/td>\n~50 million<\/td>\n<\/tr>\n
Illumina\u00ae i100 MiSeq 50M<\/td>\n100, 300, 600<\/td>\n~100 million<\/td>\n<\/tr>\n
IIllumina\u00ae i100 MiSeq 100M<\/td>\n100, 300<\/td>\n~200 million<\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq 6000 SP<\/td>\n100, 200, 300<\/td>\n~1.6 billion  <\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq 6000 S1<\/td>\n100, 200, 300<\/td>\n~3.2 billion<\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq 6000 S2<\/td>\n100, 200, 300<\/td>\n~8.2 billion<\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq 6000 S4<\/td>\n35, 200, 300<\/td>\n~20 billion<\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq X Plus 1.5B<\/td>\n100, 200, 300<\/td>\n~3 billion<\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq X Plus 10B<\/td>\n100, 200, 300<\/td>\n~20 billion<\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq X Plus 25B<\/td>\n300<\/td>\n~50 billion<\/td>\n<\/tr>\n
Element AVITI24\u2122 Cloudbreak Freestyle Low Output<\/td>\n150, 300<\/td>\n~150 million for 150 cycle
~375 million for 300 cycle<\/td>\n<\/tr>\n
Element AVITI24\u2122 Cloudbreak Freestyle Mid Output<\/td>\n150, 300, 600<\/td>\n~750 million
*600 cycle kit ~100 million<\/td>\n<\/tr>\n
Element AVITI24\u2122 Cloudbreak Freestyle High Output<\/td>\n150, 300, 600<\/td>\n~1.5 billion
*600 cycle kit ~300 million<\/td>\n<\/tr>\n
Element AVITI24\u2122 Cloudbreak Freestyle UltraQ<\/td>\n300<\/td>\n~800 million<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n
\n\n\n\n\n\n
Long-Read Sequencing Platform<\/strong><\/td>\nFlowcell Minimum<\/strong><\/td>\nOutput (Gb)<\/strong><\/td>\n<\/tr>\n
PacBio Revio\u2122<\/td>\n1 SMRTcell
(Run Time: 12, 24, or 30hrs)<\/td>\n		~60-90<\/td>\n<\/tr>\n
Oxford Nanopore Technologies\u00ae<\/td>\n1 flowcell<\/td>\n~50-150<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n","protected":false},"excerpt":{"rendered":"

Sequencing Platform Cycles (bp) Number of Reads*paired-end IIllumina\u00ae i100 MiSeq 5M 300, 500 ~10 million Illumina\u00ae i100 MiSeq 25M 100, 300, 600, 1000 ~50 million Illumina\u00ae i100 MiSeq 50M 100, 300, 600 ~100 million IIllumina\u00ae i100 MiSeq 100M 100, 300 ~200 million Illumina\u00ae NovaSeq 6000 SP 100, 200, 300 ~1.6 billion   Illumina\u00ae NovaSeq 6000 S1 100, 200, 300 ~3.2 billion Illumina\u00ae NovaSeq 6000 S2 100, 200, 300 ~8.2 billion Illumina\u00ae NovaSeq 6000 S4 35, 200, 300 ~20 billion Illumina\u00ae…<\/p>\n

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