{"id":411,"date":"2024-04-25T20:14:03","date_gmt":"2024-04-25T20:14:03","guid":{"rendered":"https:\/\/winterdev.cc\/nwgc-dev\/?page_id=411"},"modified":"2024-04-25T20:14:03","modified_gmt":"2024-04-25T20:14:03","slug":"library-prep","status":"publish","type":"page","link":"https:\/\/nwgc.gs.washington.edu\/wordpress\/services\/structural-genomics\/short-read\/wgs\/library-prep\/","title":{"rendered":"Library Prep"},"content":{"rendered":"<p class=\"has-small-font-size\">The NWGC uses KAPA Hyper PCR-Free Library Preparation Kits for whole genome sequencing. The KAPA Hyper prep offers the highest quality PCR-free sequencing libraries for samples that meet quality and quantity standards<strong>.<\/strong>  It also results in greater uniformity of coverage, lower duplicate rates, and lower GC bias compared to libraries that have been subjected to PCR.<\/p>\n<p><a href=\"https:\/\/rochesequencingstore.com\/catalog\/kapa-hyper-prep-kit-pcr-free\/\">KAPA Hyper PCR-Free Library Prep Kit<\/a><\/p>\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1200\" height=\"1200\" src=\"https:\/\/winterdev.cc\/nwgc-dev\/wp-content\/uploads\/2024\/06\/image-3.png\" alt=\"\" class=\"wp-image-853\" style=\"width:347px;height:auto\" srcset=\"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-content\/uploads\/2024\/06\/image-3.png 1200w, https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-content\/uploads\/2024\/06\/image-3-300x300.png 300w, https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-content\/uploads\/2024\/06\/image-3-1024x1024.png 1024w, https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-content\/uploads\/2024\/06\/image-3-150x150.png 150w, https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-content\/uploads\/2024\/06\/image-3-768x768.png 768w, https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-content\/uploads\/2024\/06\/image-3-375x375.png 375w\" sizes=\"auto, 100vw\" \/><\/figure>\n<h2 class=\"wp-block-heading has-small-font-size\"><strong>Alternative Low Input DNA \u2013 KAPA Hyper Prep Kit with PCR<\/strong><\/h2>\n<p class=\"has-small-font-size\"><span id=\"docs-internal-guid-1d737588-7fff-d8cf-2b48-469cb5f24fa7\"><span style=\"font-size: 11pt; font-family: Arial, sans-serif; background-color: transparent; font-variant-numeric: normal; font-variant-east-asian: normal; font-variant-alternates: normal; font-variant-position: normal; vertical-align: baseline;\">We can also offer the KAPA Hyper kit with PCR to generate libraries. The added PCR steps result in higher duplicate rates and bias in GC-rich genomic regions; however, the amount of DNA input for this library preparation method is also considerably lower.\u00a0<\/span><span style=\"font-size: 11pt; font-family: Arial, sans-serif; background-color: transparent; font-variant-numeric: normal; font-variant-east-asian: normal; font-variant-alternates: normal; font-variant-position: normal; vertical-align: baseline;\"><\/span><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The NWGC uses KAPA Hyper PCR-Free Library Preparation Kits for whole genome sequencing. The KAPA Hyper prep offers the highest quality PCR-free sequencing libraries for samples that meet quality and quantity standards. It also results in greater uniformity of coverage, lower duplicate rates, and lower GC bias compared to libraries that have been subjected to PCR. KAPA Hyper PCR-Free Library Prep Kit Alternative Low Input DNA \u2013 KAPA Hyper Prep Kit with PCR We can also offer the KAPA Hyper&#8230;<\/p>\n<div><a class=\"more-link\" href=\"https:\/\/nwgc.gs.washington.edu\/wordpress\/services\/structural-genomics\/short-read\/wgs\/library-prep\/\">Continue reading <span class=\"screen-reader-text\">Library Prep<\/span><\/a><\/div>\n","protected":false},"author":2,"featured_media":0,"parent":326,"menu_order":4,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-411","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/411","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/comments?post=411"}],"version-history":[{"count":0,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/411\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/326"}],"wp:attachment":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/media?parent=411"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}