{"id":397,"date":"2024-04-25T20:07:16","date_gmt":"2024-04-25T20:07:16","guid":{"rendered":"https:\/\/winterdev.cc\/nwgc-dev\/?page_id=397"},"modified":"2026-03-04T17:40:15","modified_gmt":"2026-03-04T17:40:15","slug":"sequencing","status":"publish","type":"page","link":"https:\/\/nwgc.gs.washington.edu\/wordpress\/services\/functional-genomics\/rnaseq\/sequencing\/","title":{"rendered":"Sequencing – Available Platforms"},"content":{"rendered":"
\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Sequencing Platform<\/strong><\/td>\nCycles (bp)<\/strong><\/td>\nNumber of Reads
*paired-end<\/strong><\/td>\n<\/tr>\n
Illumina\u00ae MiSeq<\/td>\n50, 300, 500<\/td>\n~30 million for V2 ~50 million for V3<\/td>\n<\/tr>\n
Illumina\u00ae NextSeq 500 MO<\/td>\n75, 150, 300<\/td>\n~260 million<\/td>\n<\/tr>\n
Illumina\u00ae NextSeq 500 HO<\/td>\n75, 150, 300<\/td>\n~400 million<\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq 6000 SP<\/td>\n100, 200, 300<\/td>\n~1.6 billion  <\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq 6000 S1<\/td>\n100, 200, 300<\/td>\n~3.2 billion<\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq 6000 S2<\/td>\n100, 200, 300<\/td>\n~8.2 billion<\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq 6000 S4<\/td>\n35, 200, 300<\/td>\n~20 billion<\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq X Plus 1.5B<\/td>\n100, 200, 300<\/td>\n~3 billion<\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq X Plus 10B<\/td>\n100, 200, 300<\/td>\n~20 billion<\/td>\n<\/tr>\n
Illumina\u00ae NovaSeq X Plus 25B<\/td>\n300<\/td>\n~50 billion<\/td>\n<\/tr>\n
Element AVITI\u2122 Cloudbreak Freestyle Low Output<\/td>\n150, 300<\/td>\n~150 million for 150 cycle
~375 million for 300 cycle<\/td>\n<\/tr>\n
Element AVITI\u2122 Cloudbreak Freestyle Mid Output<\/td>\n150, 300, 600<\/td>\n~500 million *600 cycle kit ~100 ~750 million
*600 cycle kit ~100 million<\/td>\n<\/tr>\n
Element AVITI\u2122 Cloudbreak Freestyle High Output<\/td>\n150, 300, 600<\/td>\n~1.5 billion
*600 cycle kit ~300 million<\/td>\n<\/tr>\n
Element AVITI24\u2122 Cloudbreak Freestyle UltraQ<\/td>\n300<\/td>\n~800 million<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n
\n\n\n\n\n\n
Long-Read Sequencing Platform<\/strong><\/td>\nFlowcell Minimum<\/strong><\/td>\nOutput (Gb)<\/strong><\/td>\n<\/tr>\n
PacBio Revio\u2122<\/td>\n1 SMRTcell
(Run Time: 12, 24, or 30hrs)<\/td>\n		~60-90<\/td>\n<\/tr>\n
Oxford Nanopore Technologies\u00ae<\/td>\n1 flowcell<\/td>\n~50-150<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n","protected":false},"excerpt":{"rendered":"

Sequencing Platform Cycles (bp) Number of Reads*paired-end Illumina\u00ae MiSeq 50, 300, 500 ~30 million for V2 ~50 million for V3 Illumina\u00ae NextSeq 500 MO 75, 150, 300 ~260 million Illumina\u00ae NextSeq 500 HO 75, 150, 300 ~400 million Illumina\u00ae NovaSeq 6000 SP 100, 200, 300 ~1.6 billion   Illumina\u00ae NovaSeq 6000 S1 100, 200, 300 ~3.2 billion Illumina\u00ae NovaSeq 6000 S2 100, 200, 300 ~8.2 billion Illumina\u00ae NovaSeq 6000 S4 35, 200, 300 ~20 billion Illumina\u00ae NovaSeq X Plus 1.5B…<\/p>\n

Continue reading Sequencing – Available Platforms<\/span><\/a><\/div>\n","protected":false},"author":2,"featured_media":0,"parent":322,"menu_order":5,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-397","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/397","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/comments?post=397"}],"version-history":[{"count":1,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/397\/revisions"}],"predecessor-version":[{"id":2473,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/397\/revisions\/2473"}],"up":[{"embeddable":true,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/322"}],"wp:attachment":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/media?parent=397"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}