{"id":379,"date":"2024-04-25T20:04:19","date_gmt":"2024-04-25T20:04:19","guid":{"rendered":"https:\/\/winterdev.cc\/nwgc-dev\/?page_id=379"},"modified":"2024-04-25T20:04:19","modified_gmt":"2024-04-25T20:04:19","slug":"quality-control","status":"publish","type":"page","link":"https:\/\/nwgc.gs.washington.edu\/wordpress\/services\/functional-genomics\/fiberseq\/quality-control\/","title":{"rendered":"Quality Control"},"content":{"rendered":"<h2 class=\"wp-block-heading\">Pre-GEM<\/h2>\n<p>Check nuclear morphology for high-quality nuclei.<\/p>\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"775\" height=\"374\" src=\"https:\/\/winterdev.cc\/nwgc-dev\/wp-content\/uploads\/2024\/08\/image-18.png\" alt=\"\" class=\"wp-image-1427\" srcset=\"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-content\/uploads\/2024\/08\/image-18.png 775w, https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-content\/uploads\/2024\/08\/image-18-300x145.png 300w, https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-content\/uploads\/2024\/08\/image-18-768x371.png 768w, https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-content\/uploads\/2024\/08\/image-18-375x181.png 375w\" sizes=\"auto, 100vw\" \/><\/figure>\n<h2 class=\"wp-block-heading\">Post-cDNA Amplification (for Gene Expression Library Prep Only)<\/h2>\n<p>An aliquot is run on the Bioanalyzer to calculate the amount of cDNA to move into the 3&#8242; Gene Expression Library Construction step.<\/p>\n<h2 class=\"wp-block-heading\">Post-Library Prep<\/h2>\n<p>Final libraries are run on qPCR and the Bioanalyzer to gather concentration and insert size information, respectively. <\/p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Pre-GEM Check nuclear morphology for high-quality nuclei. Post-cDNA Amplification (for Gene Expression Library Prep Only) An aliquot is run on the Bioanalyzer to calculate the amount of cDNA to move into the 3&#8242; Gene Expression Library Construction step. Post-Library Prep Final libraries are run on qPCR and the Bioanalyzer to gather concentration and insert size information, respectively.<\/p>\n<div><a class=\"more-link\" href=\"https:\/\/nwgc.gs.washington.edu\/wordpress\/services\/functional-genomics\/fiberseq\/quality-control\/\">Continue reading <span class=\"screen-reader-text\">Quality Control<\/span><\/a><\/div>\n","protected":false},"author":2,"featured_media":0,"parent":320,"menu_order":3,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-379","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/379","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/comments?post=379"}],"version-history":[{"count":0,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/379\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/320"}],"wp:attachment":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/media?parent=379"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}