{"id":324,"date":"2024-04-25T19:42:19","date_gmt":"2024-04-25T19:42:19","guid":{"rendered":"https:\/\/winterdev.cc\/nwgc-dev\/?page_id=324"},"modified":"2026-03-04T17:23:10","modified_gmt":"2026-03-04T17:23:10","slug":"short-read","status":"publish","type":"page","link":"https:\/\/nwgc.gs.washington.edu\/wordpress\/services\/structural-genomics\/short-read\/","title":{"rendered":"Short Read Sequencing &#8211; DNA"},"content":{"rendered":"<p>Short-read sequencing is the most commonly used form of Next-Generation Sequencing (NGS). It has a wide range of diagnostic applications, including whole genome sequencing, whole exome sequencing, RNAseq, gene panel testing, and single-gene testing. In short-read sequencing, the genome is fragmented into small fragments (typically 200-300bp) before being sequenced. Post-sequencing, alignment to reference genomes, variant calling, and variant annotation can be performed.<\/p>\n<h2 class=\"wp-block-heading\">Illumina Sequencing By Synthesis Chemistry <\/h2>\n<p><strong>(MiSeq, NextSeq, NovaSeq6000)<\/strong><\/p>\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\">\n<div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"Overview of Illumina Sequencing by Synthesis Workflow | Standard SBS chemistry\" width=\"720\" height=\"405\" src=\"https:\/\/www.youtube.com\/embed\/fCd6B5HRaZ8?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div>\n<\/figure>\n<h2 class=\"wp-block-heading\">Illumina XLEAP Chemistry <\/h2>\n<p><strong>(NovaSeq X)<\/strong><\/p>\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\">\n<div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"Illumina - XLEAP-SBS\u2122 Chemistry\" width=\"720\" height=\"405\" src=\"https:\/\/www.youtube.com\/embed\/ppiW7hAvel8?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div>\n<\/figure>\n<h2 class=\"wp-block-heading\">Element Avidite Chemistry<\/h2>\n<p><strong>(AVITI24)<\/strong><\/p>\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube\">\n<div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"Element Biosciences: Avidity Sequencing \u2013 Accuracy and Applications\" width=\"720\" height=\"405\" src=\"https:\/\/www.youtube.com\/embed\/cWr4m7fCnzc?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div>\n<\/figure>\n","protected":false},"excerpt":{"rendered":"<p>Short-read sequencing is the most commonly used form of Next-Generation Sequencing (NGS). It has a wide range of diagnostic applications, including whole genome sequencing, whole exome sequencing, RNAseq, gene panel testing, and single-gene testing. In short-read sequencing, the genome is fragmented into small fragments (typically 200-300bp) before being sequenced. Post-sequencing, alignment to reference genomes, variant calling, and variant annotation can be performed. Illumina Sequencing By Synthesis Chemistry (MiSeq, NextSeq, NovaSeq6000) Illumina XLEAP Chemistry (NovaSeq X) Element Avidite Chemistry (AVITI24)<\/p>\n<div><a class=\"more-link\" href=\"https:\/\/nwgc.gs.washington.edu\/wordpress\/services\/structural-genomics\/short-read\/\">Continue reading <span class=\"screen-reader-text\">Short Read Sequencing &#8211; DNA<\/span><\/a><\/div>\n","protected":false},"author":2,"featured_media":0,"parent":304,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-324","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/324","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/comments?post=324"}],"version-history":[{"count":2,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/324\/revisions"}],"predecessor-version":[{"id":2468,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/324\/revisions\/2468"}],"up":[{"embeddable":true,"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/pages\/304"}],"wp:attachment":[{"href":"https:\/\/nwgc.gs.washington.edu\/wordpress\/wp-json\/wp\/v2\/media?parent=324"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}