As part of the All of Us Research Program, the NWGC has produced high quality PacBio HiFi data for hundreds of samples. We initially used the Sequel IIe platform, but have since upgraded to the Revio, which allows us to simultaneously run four SMRTcells. Each SMRTcell can produce more than 100Gb of long read data with more than 92% of bases exceeding Q30. The Revio can sequence up to 16 SMRT cells per week, making it an efficient system for long read sequencing.
Below is a list of important information and tips for using our Library Construction and Sequencing services.
1.) Have a clear outline of the goals and expectations for your project. The project managers in our lab will do everything they can to make sure these are met, and having this information upfront can greatly help with providing all the data and information you need.
2.) Do not submit samples without first contacting us for a quote and a project manager sends you a blank manifest (sample sheet) to fill out. For quotes, please email nwgc@uw.edu.
3.) Please review the Submitting Samples instructions carefully! This document has detailed information about how our processes and pipelines function, so it is important to review prior to submitting samples.
4.) If you have any questions during the process, the project manager is the best contact to answer them. Please see the Contact Us page.