Typically, 350 million paired-end reads are needed for each scATAC-seq library and 250 million paired-end reads for each scRNA-seq library. Depending on how many libraries are pooled together, the kit size will vary.
Sequencing Platform | Cycles (bp) | Number of Reads *paired-end |
Illumina® MiSeq | 50, 300, 500 | ~30 million for V2 ~50 million for V3 |
Illumina® NextSeq 500 MO | 75, 150, 300 | ~260 million |
Illumina® NextSeq 500 HO | 75, 150, 300 | ~400 million |
Illumina® NovaSeq 6000 SP | 100, 200, 300 | ~1.6 billion |
Illumina® NovaSeq 6000 S1 | 100, 200, 300 | ~3.2 billion |
Illumina® NovaSeq 6000 S2 | 100, 200, 300 | ~8.2 billion |
Illumina® NovaSeq 6000 S4 | 35, 200, 300 | ~20 billion |
Illumina® NovaSeq X Plus 1.5B | 100, 200, 300 | ~3 billion |
Illumina® NovaSeq X Plus 10B | 100, 200, 300 | ~20 billion |
Illumina® NovaSeq X Plus 25B | 300 | ~50 billion |
Element AVITI™ Cloudbreak Freestyle Low Output | 300 | ~250 million |
Element AVITI™ Cloudbreak Freestyle Mid Output | 150, 300, 600 | ~500 million *600 cycle kit ~100 million |
Element AVITI™ Cloudbreak Freestyle High Output | 150, 300, 600 | ~1 billion *600 cycle kit ~300 million |