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Sample Prep & Sequencing: Next Generation Sequencing

The NWGC utilizes Illumina sequencing instruments, which employ sequencing by synthesis technology. This method of sequencing allows for massive parallel sequencing to take place in an efficient and cost effective manner.

The NWGC currently operates:

  • MiSeqs
  • NextSeq500
  • HiSeq 4000s featuring patterned flow cells
  • HiSeq Xs featuring patterned flow cells
  • NovaSeq 6000s featuring patterned flow cells
PlatformReads per Lane*Max Read LengthLanes per flow cellRun Time
MiSeq~15 million for v2 & ~25 million for v3up to PE25011-2 Days
NextSeq~130 million for mid-output & ~400 million for high-outputup to PE15011-2 Days
HiSeq 4000~312 millionup to PE150**82-3 Days
HiSeq X~375 millionPE15183 Days
NovaSeq 6000~3.3 billion for S2 flow cells & ~10 billion for S4 flow cellsup to PE15111-2 Days

* Number of reads is an estimate, and can be higher or lower based on library prep factors and clustering

** These instruments are only run as PE75 unless you purchase an entire flow cell