Sample Prep & Sequencing: Library Prep
The Northwest Genomics Center has extensive experience in exome, custom target, whole genome and RNA library preparation. Our automated, high-throughput methods allow us to process hundreds of samples per week without sacrificing quality and precision of results. All samples are subject to quality control testing at multiple stages to monitor sample integrity.
Our most common prep method is Nimblegen SeqCap Human Exome v2.0, which uses over 2 million probes to capture 20,000 genes. We also utilize Nimblegen SeqCap Human Exome v3.0 and SeqCap EZ MedExome prep methods.
The NWGC commonly uses TruSeq Stranded mRNA Library Prep Kits, however we have the capacity to prepare samples using many other RNA kits to meet your needs.
Whole Genome Sequencing:
The NWGC uses KAPA Hyper PCR-Free Library Preparation Kits for whole genome sequencing.
Nimblegen NimbleDesign allows researchers to create a set of probes to capture a specific region on the genome.
For low input samples, the NWGC utilizes KAPA HTP Library Preparation Kits.